Synergistic interaction of Piriformospora indica and microbial inoculants on symbiotic potential, plant nutrition and productivity of chickpea (Cicer arietinum)
DOI:
https://doi.org/10.59797/ija.v62i4.5555Keywords:
Chickpea, Leghemoglobin, Mesorhizobium ciceri, Nitrogenase activity, Nutrient availability, Piriformospora indicaAbstract
The present study was carried out during the winter season of 2011–12 and 2012–13 at Indian Agricultural Re search Institute, New Delhi, to evaluate the efficacy of microbial bioinoculants in chickpea (Cicer arietinum L.) us ing combinatorial approach. A group of microbial inoculants (Mesorhizobium ciceri). Phosphate-solubilizing micro organisms and plant growth-promoting rhizobacteria) in presence and absence of P fertilizer was used to testify its synergistic effect with Piriformospora indica on symbiotic traits, growth and yield of chickpea under field condi tions. Significant differences were observed with respect to symbiotic potential and other associated parameters with combined inoculation. Inoculation with Mesorhizobium ciceri enhanced nitrogenase activity and leghemoglobin content significantly over un-inoculated control. Significant improvement in N and P nutrition with combined inoculation amended with 30 kg P/ha emphasized the beneficial effect of these and similar trend was reflected in productivity enhancement as well.
References
Appleby, C.A. and Bergersen, F.J. 1980. Preparation and experimental use of leghaemoglobin. Methods for Evaluating Biological Nitrogen Fixation. pp. 315–335. (In) Bergersen, F.J. (Ed.) John Wiley & Sons, New York.
Barea, J.M., Toro, M., Orozco, M.O., Campos, E. and Azcon, R. 2002. The application of isotopic (32P and 15N) dilution techniques to evaluate the interactive effect of phosphate-solubilizing rhizobacteria, mycorrhizal fungi and Rhizobium to improve the agronomic efficiency of rock phosphate for legume crops. Nutrient Cycling in Agroecosystems 63(1): 35–42.
Bashan, Y. and de-Bashan, L.E. 2010. How the plant growth-promoting bacterium Azospirillum promotes plant growth – a critical assessment. Advances in Agronomy 108: 77–136.
Downie, J.A. 2005. Legume haemoglobins: symbiotic nitrogen fixation needs bloody nodules. Current Biology 15(6): 196–198.
Dupont, L., Herouart, D., Alloing, G., Hopkins, J., Pierre, O., Frendo, P. and El Msehli, S. 2012. The legume root nodule: from symbiotic nitrogen fixation to senescence. (In) Nagata, T. (Ed.) Senescense, In Tech, pp. 137–168.
Franken, F. 2012. The plant strengthening root endophyte Piriformospora indica: potential application and the biology behind. Applied Microbiology and Biotechnology 96: 1,455–1,464.
Graham, P.H. 1985. Problems of soybean inoculation in the tropics. (In) World Soybean Research Conference III: Proceedings. Shibles, R. (Ed.) Westview Press, Boulder, USA, pp. 951–959.
Hardy, R.W.F., Burns, R.C. and Holsten, R.D. 1973. Application of acetylene-ethylene assay for measurement of nitrogen fixation. Soil Biology and Biochemistry 5: 47–81.
Hiscox, J.D. and Israelstam, G.F. 1979. A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany 57: 1,332–1,334.
Korir, H., Mungai, N.W., Thuita, M., Hamba, Y., and Masso, C. 2017. Co-inoculation effect of rhizobia and plant growth promoting rhizobacteria on common bean growth in a low phosphorus soil. Frontiers in Plant Science 8: 141. doi: 10.3389/fpls.2017.00141
Leidi, E.O. and Rodriguez-Navarro, D.N. 2000. Nitrogen and phosphorus availability limit N2 fixation in bean. New Phytologist 147: 337–346.
Lupwayi, N.Z., Clayton, G.W. and Rice, W.A. 2006. Rhizobial inoculants for legume crops. Journal of Crop Improvement 15(2): 289–321.
Mansotra, P., Sharma, P. and Sharma, S. 2015. Bioaugmentation of Mesorhizobium ciceri, Pseudomonas spp. and Piriformospora indica for sustainable chickpea production. Physiology and Molecular Biology of Plants 21(3) : 385–393.
Neill, S., Desikan, R. and Hancock, J. 2002. Hydrogen peroxide signaling. Current Opinion in Plant Biology 5: 388–393.
Neo, H.H. and Layzell, D.B. 1997. Phloem glutamine and the regulation of O2 diffusion in legume nodules. Plant Physiology 113: 259–267.
Nour, S.M., Fernandez, M.P., Normand, P. and Cleyet-Marel, J.C. 1994. Rhizobium ciceri sp. nov. consisting of strains that nodulate chickpeas (Cicer arietinum L.). International Journal of Systematic Bacteriology 44: 511–522.
Prasad, R., Pathak, H., Patra, A.K. and Shivay, Y.S. 2014. Nitrogen management (In) Textbook of Plant Nutrient Management, pp 73–92. Prasad, R., Kumar, D., Rana, D.S., Shivay, Y.S. and Tewatia, R.K. (Eds.) Indian Society of Agronomy, Indian Agricultural Research Institute, New Delhi.
Prasad, R., Shivay, Y.S. and Kumar, D. 2013. A Practical Manual of Analytical Methods for Soil and Plant Samples from Agronomy Field Experiments, pp. 1–58. Venus Printers & Publishers, New Delhi.
Riggs, P.J., Chelius, M.K., Iniguez, A.L., Kaeppler, S




